Radioimmunoassay of succinyl cyclic AMP and application to micro-determination of cyclic nucleotides in blood

Enzymatic radioiodination of succinyl cyclic AMP tyrosine methyl ester by lactoperoxidase and radioimmunoassay for cyclic AMP.

A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and...

Radioimmunoassay for Cyclic Nucleotides

Sensitive and specific radioimmunoassays have been developed for cyclic adenosine 3’, 5’-monophosphate (CAMP), cyclic guanosine 3’ ,5’-monophosphate (cGMP), cyclic inosine 3’ ,5’-monophosphate (cIMP), and cyclic uridine 3’, 5’monophosphate (cUMP). These assays are based upon competition of the cyclic nucleotide with isotopically labeled cyclic nucleotide derivatives for binding sites on specifi...

application and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples

ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...

آدنوزین منوفسفات حلقوی ( Cyclic AMP )

Cyclic nucleotides and thyroid hormones: radioimmunoassay or protein-binding assay?